细胞生物学试剂

【现货日本和光WAKO细胞抑制剂价格优惠保证质量】

NK细胞抑制剂 Anti asialo GM1 (Rabbit)NK细胞抑制剂Anti Asialo GM1(Anti AsGM1)本产品只能用于研究,不能用于人的治疗。◆原理介绍   Anti Asialo GM1(Rabbit)抗血清可以减少大鼠或者小鼠来源的自然杀伤细胞(NK细胞)的活性,NK细胞是免疫监视细胞。该抗体可以与NK细胞发生特异性结合,从而抑制NK细胞非特异性杀伤靶细胞的活性。靶

  • 进口/国产: 现货日本和光WAKO细胞抑制剂价格优惠保证质量
  • 产地/品牌: 现货日本和光WAKO细胞抑制剂价格优惠保证质量
  • 型号/货号: 现货日本和光WAKO细胞抑制剂价格优惠保证质量
  • 报价/价格: 现货日本和光WAKO细胞抑制剂价格优惠保证质量
  • 供应商: 北京微宣科技有限公司
  • 规格/数量: 现货日本和光WAKO细胞抑制剂价格优惠保证质量

NK细胞抑制剂 Anti asialo GM1 (Rabbit)

NK细胞抑制剂Anti Asialo GM1(Anti AsGM1)新品速递图wako.jpg

产品只能用于研究,不能用于人的治疗。

原理介绍

   Anti Asialo GM1(Rabbit)抗血清可以减少大鼠或者小鼠来源的自然杀伤细胞(NK细胞)的活性,NK细胞是免疫监视细胞。该抗体可以与NK细胞发生特异性结合,从而抑制NK细胞非特异性杀伤靶细胞的活性。靶细胞包括肿瘤细胞、同种异体移植的器官、组织等。可运用于药物筛选,器官移植,自身免疫疾病等研究。

  每个产品有具体的体内外的滴定数据。

◆注射方法

小鼠-静脉注射:

10-50μL(推荐20μL)具体剂量由滴度和规格而定。第一次注射在4天内有效,因此两周内需要注射3-4次。

大鼠-静脉注射:

50-250μL(小鼠剂量的4或5倍)具体剂量还须考虑大鼠的健康状况、体重和NK活性。

小鼠和大鼠-腹腔给药:

同等剂量或多于静脉注射。

产品信息

 

制备

 

从牛脑组织里提纯的Asialo GM1与甲基化牛血清白蛋白和弗氏完全佐剂一起使用,可进行反复免疫。用含有磷酸缓冲盐水(ph7.2)的50%硫酸铵透析后,得到血清里的丙种球蛋白(Gammaglobulin)片段。

内容

含有唾液酸抗体GM1的兔血清50%硫酸铵盐析分层物(含蛋白量约10mg/mL)。

提纯法

50%的硫酸铵盐析后 ,用含有磷酸缓冲液的生理盐水透析,然后冻干。

特异性

作用于小鼠和大鼠的自然杀伤细胞;小鼠单核细胞(是一种不含自然杀伤细胞、骨髓、胎儿肝细胞和裸鼠脾脏巨噬细胞的肝细胞);小鼠胎儿胸腺细胞(12日龄;生存率不断降低直到没有出现新生小鼠)(备注:如果浓度高,也能作用于成熟的T淋巴细胞)

免疫球蛋白类型

IgG、IgA和IgM

重组

建议用蒸馏水(1mL)。由于该产品是使用盐来进行冻干的,如果用其它溶剂如PBS或MEM,就能增加其盐浓度。

抗体滴度

通过免疫凝聚实验得出结果,抗体滴度比例约1 : 1,000

保存方法

存储在2~10℃下

包装规格

1mL样瓶

形状

干冻品

应用案例

适用的动物模型

运用

肿瘤动物模型

与抗肿瘤药物混合后注入肿瘤动物模型内,研究抗肿瘤药物的作用机理。

肝纤维化动物模型

与治疗肝纤维化的药物混合后注入动物模型体内,研究抗肝纤维化药物的作用机理。

器官移植动物模型

器官移植后,会产生免疫排斥反应,机体内的免疫监视会清除”异己“的细胞和器官。可用于研究器官移植后使用的免疫抑制剂的作用机理

自身免疫疾病动物模型

研究治疗自身免疫疾病药物的作用机理。

干细胞动物模型

抑制NK细胞的活性,从而有利于干细胞动物模型的建立。

糖尿病动物模型

研究炎症与糖尿病的关系。

详情请点NK细胞抑制剂——Anti Asialo GM1(Anti AsGM1)(兔源)

参考文献

[1] Nishikado H, Mukai K, Kawano Y, et al. NK cell-depletinganti-asialo GM1 antibody exhibits a lethal 

[3] off-target effect on basophils invivo[J]. The Journal of Immunology, 2011, 186(10): 5766-5771.

[2] Kasai, M., Iwamori, M., Nagai, Y., Okumura, K. and Tada, T.: Eur. J. Immunol., 10, 175 (1980)

[3] Habu, S., Fukui, H., Shimamura, M., Kasai, M., Nagai, Y., Okumura, K. and Tamaoki, N.: Immunol., 

[2] 127, 34 (1981)

[4] Jinxing Xia et al., Clinica l Immunology.(2010) 134, 130–139.

[5] Eirini Christaki et al., Journal of Immunology Research, Volume 2015 (2015), Article ID 532717,

[5] 10 pages.

[6] Hata R et al.,Scientific Reports(2015) 5 : 9083 | DOI: 10.1038/srep09083


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145-06381NICARDIPINE HYDROCHLORIDE1G
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147-07201NAPROXEN5G
148-04791NALIDIXIC ACID5G
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134-10081MINOCYCLINE HYDROCHLORIDE1G
131-09403MIDECAMYCINE1G
135-09401MIDECAMYCINE250MG
132-12662MICONAZOLE NITRATE25G
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065-01732FRADIOMYCIN SULFATE25G
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014-11913AMIKACINE SULFATE5G
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019-13543AA-86110MG
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