Certificate of Analysis and Data Sheet
Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E.coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin).
Recombinant Staphylococal Protein A produced in E.Coli is a non-glycosylated, Polypeptide chain containing 178 amino acids and having a molecular mass of 23,307 Dalton.
Recombinant Staphylococal Protein A is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
Recombinant Protein A was lyophilized from a concentrated (1mg/ml) solution containing no additives.
Recombinant Protein A although stable at 4 C for 21 days, should be stored desiccated below -20 C for long term periods .
Please avoid freeze-thaw cycles.
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Dimers and aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Less than 0.1 ng/µg (IEU/µg) of Recombinant Protein A.
Greater than 95% binding activity to human IgG.
Prospec''s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Immunoglobulin G-binding protein A precursor (IgG-binding protein A)
(Staphylococcal protein A).